Physical Genome Map of the Unicellular Cyanobacterium Synechococcus sp. Strain PCC 7002

Xiangning Chen and William R. Widger*

Department of Biochemical and Biophysical Sciences, University of Houston, Houston, Texas 77204-5934

Received 24 March 1993/Accepted 24 May 1993

ABSTRACT

A physical restriction map of the genome of the cyanobacterium Synechococcus sp. strain PCC 7002 was assembled from AscI, NotI, SalI, and SfiI digests of intact genomic DNA separated on a contour-clamped homogeneous electric field pulsed-field gel electrophoresis system. An average genome size of 2.7 x 10⁶ bp was calculated from 21 NotI, 37 SalI, or 27 SfiI fragments obtained by the digestions. The genomic map was assembled by using three different strategies: linking clone analysis, pulsed-filed fragment hybridization, and individual clone hybridization to singly and doubly restriction digested large fragments. The relative positions of 21 genes or operons were determined, and these data suggeste that gene order is not highly conserved between Synechococcus sp. strain PCC 7002 and Anabaena sp. strain PCC 7120.

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