A schematic drawing illustrates the TDI assay. After PCR ampalificaiton of target DNA segament, excess PCR primers and dNTPs are digested with exonucease I and alkaline phosphotase. Heat inactivation is necessay to guarentee the success of primer extention reaction, which is the allelic discrimination reaction. The result of this reaction can be inferred by monitoring FRET.

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Last revised: January 13, 2004.