A scheme illustrates the principle of fluorescence resonance energy transfer(FRET). When two fluorephores are in close proximaty and one fluorephore's emmision spectrum (the donor, D) overlaps the other's (the acceptor, A) excitation spectrum, FRET will occur. When FRET occurs, spectroscopically, the donor's intensity decreases while the acceptor's intensity increases. We take the advantage of this phenomenon as our detection method for allelic discrimination reaction. During this reaction, when two fluorescence dye labeled oligonucleotides are chemically linked together, FRET will be observed. FRET, therefore, could be used as a index for the allelic discriminaiton reaction.

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Last revised: January 13, 2004.